Moffitt et al (2016): Development of a Novel ProteaseTag® Activity Immunoassay for the Specific Measurement of Neutrophil Elastase in Clinical Samples from Patients with Respiratory Disease. Lab Methodology and Engineering A7425-A7425
Moffitt et al (2015) Development of a Novel Assay for the Detection of Active Neutrophil Elastase in Patients with Chronic Obstructive Pulmonary Disease, Thorax;70:A126-A127: The objective of this study was to clinically validate ProteaseTag® Active NE Ultra Immunoassay for the detection of NE in sputum from COPD patients.
Moffitt et al (2015) Utilisation of a novel ProteaseTag™; activity immunoassay for the specific measurement of neutrophil elastase in BAL from children with cystic fibrosis. Pediatr Pulmonol (s41) p266-267
Korkmaz et al. (2010) Neutrophil Elastase, Proteinase 3, and Cathepsin G as Therapeutic Targets in Human Diseases. Pharmacological Reviews 62 (4) 726-759.
Key clinical studies
Sly et al (2013) Risk factors for bronchiectasis in children with cystic fibrosis. New Engl J Med 368: 1963-1970. This longitudinal study in clinically stable children over the first three years of life found free NE in bronchoalveolar lavage at 3 months to be the major predictor of persistent bronchiectasis at both 12 months and 3 years of age.
Sagel et al (2012) Sputum biomarkers of inflammation and lung function decline in children with cystic fibrosis. Am J Respir Crit Care Med 186: 857-865. This prospective, longitudinal cohort study, conducted over three years in 35 CF children and measuring a range of airway mediators related to proteolysis and a panel of inflammatory cytokines concluded sputum levels of NE to be predictive of subsequent lung function decline and the most informative biomarker to monitor disease activity.
Mayer-Hamblett et al (2007) Association between pulmonary function and sputum biomarkers in cystic fibrosis. Am J Respir Crit Care Med 175: 822-828. Data from 4 multi-centre studies identified NE to negatively correlate with lung function (forced expiratory volume in 1 second; FEV1). Patients who differed in their NE measurements by 0.5 log differed in FEV1 values by -7.3%. NE was also shown to have a longitudinal association with FEV1 specifically a -0.29% decline in FEV1 per 1 log increase of NE.
A BMG Labtech application note concludes that the ProteaseTag™ Active NE Immunoassay provides a simple and quick method of assessing active neutrophil elastase levels, and constitutes a reliable method for the detection of infection and inflammation in biological samples.