ProteaseTags® inactivate a specific protease and may be used as tools to detect and quantify the activity of a single protease species within a complex biological sample.
Unlike antibodies, ProteaseTags® are selective for the active protease and are species-blind i.e. can be utilized for the detection of bacterial, viral, amphibian as well as mammalian proteases.
ProteaseTags® not only inhibit/capture active proteases, they are designed to form a bridge to a solid support allowing them to be combined with established technology platforms such as ELISA, lateral flow or multianalyte biochips.
Our assays specifically detect active proteinases without utilising chromogenic or fluorogenic substrates which are often not completely specific when added to crude biological samples containing multiple proteolytic and hydrolytic proteinases.
ProteaseTag® Technology can be applied to a broad-spectrum of disease processes in which abnormal protease activity is a key feature, such as inflammation, thrombosis, tumour invasion/metastasis and wound healing.
Validation of ProteaseTags®
ProteaseTag® technology has been validated in a wide range of complex biological fluids, including:
Clinical samples such as sputum, bronchoalveolar lavage, blood and wound exudates.
- Cell conditioned media
- Tumour homogenates
- Bacterial lysates and biofilm cultures
- Amphibian secretions and venoms